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Derwent Biotechnology Resource provides complete coverage of
biotechnology literature and patents worldwide.
DWBA contains information on all aspects of biotechnology including:
genetic engineering, biochemical engineering, pharmaceutical
technology, agriculture, cell culture, biocatalysis, waste disposal
and the environment.
Abstracts for DWBA are specially written by a subject specialist,
and each abstract contains a concise summary of methods, results and
conclusions. All abstracts are in English.
Full bibliographic details are provided including author reprint
address for literature, and full legal information for patents. A
controlled vocabulary is used for easy and accurate searching.
About one third of the database covers patents.
DWBA will be of use to research scientists in biotechnology,
agriculture and pharmaceuticals companies, in universities, research
institutes and science libraries etc.
Use DWBA to answer questions such as:
- are there any gene therapy vaccines for the treatment of AIDS or
HIV virus infection?
- which antibiotics are produced by Streptomyces sp.?
- have fermentors/digestors been developed for the disposal of
industrial waste with consequent methane production?
- what biological methods are used for bioremediation of oil spills
since the Exxon Valdez oil spill?
- have micro-organisms been used for the recovery of precious metals?
Sources:
Leading scientific and technical journals, conference proceedings
and meeting reports, and the world-wide patent literature.
Accession number & update
1998-09431 19980830.
Title
Method for the isolation of the glucose-6-phosphate-dehydrogenase
gene of Leuconostoc mesenteroides.
Patent assignee
Syracuse-Univ.
Patent number
WO9207078.
Patent publication date
30 April 1998 (19980430).
Priority information
Priority date: 12 October 1997 (19971012)
Priority number: US-596867.
Filing date: 15 October 1998 (19981015)
Filing number: 97US-WO07715.
Language
EN.
Abstract
Glucose-6-phosphate-dehydrogenase (G6PDH, EC-1.1.1.49) from
Leuconostoc mesenteroides is produced by: isolating genomic DNA
encoding G6PDH from L. mesenteroides; preparing oligonucleotides
complementary to the upstream and downstream DNA of the G6PDH
gene; subjecting the genomic DNA to the polymerase chain reaction
(PCR) with the oligonucleotides; digesting the amplified DNA with
BamHI and SphI to produce a restriction fragment; ligating the
fragment into a plasmid; introducing the plasmid into a suitable
Escherichia coli strain; and isolating G6PDH from a recombinant
E. coli culture. DNA sequences for the primers and the G6PDH gene
are specified. Cloning of the G6PDH gene from L. mesenteroides
allows sequence comparison with the equivalent gene in other
species. Such information is useful in designing site-directed
mutagenesis experiments in order to determine the enzyme
mechanism. In an example, the G6PDH gene was cloned in E. coli
DH5-alpha MCR, using plasmid pUC19 as vector. Recombinant plasmid
plmz, containing the entire gene, was isolated. The specific
activity of G6PDH was 5,000 times increased in the recombinant
strain. (22pp).
Classification codes
K BIOCATALYSIS; K1 Isolation & Characterization; A MICROBIOLOGY;
A1 Genetics.
Publication type
P PATENT.
Descriptors
Leuconostoc mesenteroides recombinant glucose-6-phosphate-
dehydrogenase prep., expression in Escherichia coli, DNA
sequence;
bacterium enzyme EC-1.1.1.49 gene cloning.
WPI number & Derwent week
92-167158; Derwent Week 20.
Labels/description Example
AN Accession number 1_: 1998-09431
& update - see Limit options -
- accession year 2_: AY=1998
TI Title 3_: LEUCONOSTOC ADJ
MESENTEROIDES.TI.
AU Author(s) 4_: MATTSSON-D$.AU.
IN Author affiliation 5_: UNIVERSITY ADJ MINNESOTA.IN.
PA Patent assignee 6_: SYRACUSE-UNIV.PA.
PN Patent number 7_: WO9207078.PN.
8_: WO.PN.
PD Patent publication date 9_: PD=19970726
PR Priority information 10_: US ADJ 596867.PR.
SO Source 11_: J-VIROL
YR Publication year 12_: YEAR=1998
- see also Notes below -
LG Language 13_: LG=EN
..ROOT LG= for a list of
available terms
AB Abstract 14_: LEUCONOSTOC ADJ MESENTEROIDES
PT Publication type
- patent-type documents 15_: PT=PATENT
- literature-type documents 16_: PT=LITERATURE
CC Classification codes 17_: CC=E5.
18_: ANIMAL ADJ CELL ADJ CULTURE
DE Descriptors 19_: LEUCONOSTOC WITH
DEHYDROGENASE.DE.
20_: GLUCOSE-6-PHOSPHATE-
DEHYDROGENASE
RF WPI Number & Derwent Week 21_: '20'.RF.
1_: YEAST.TI.
YEAR Publication year YYYY 2_: ..L 1 YEAR>1998
- see Notes below -
PD Pat. pub date YYYYMMDD 3_: ..L 1 PD>199800101
PR Pat. prio. date YYYYMMDD 4_: ..L 1 PR<199700501
FD Pat. filing date YYYYMMDD 5_: ..L 1 FD=199811213
AY Accession year YYYY 6_: ..L 1 AY=1998
UDATE Update date YYYYMMDD 7_: ..L 1 UDATE>19980630
UMONTH Update Month YYYYYYMM 8_: ..L 1 UMONTH WL 199303,199310
By paragraph - author, title: _: ..P AU, TI 1-20
SHORT AN TI AU SO PA PN
MEDIUM AN TI AU SO PA PN AB
LONG AN TI AU SO PA PN PD PR AB DE CC
ALL AN TI AU IN PA PN PD PR SO YR LG AB CC DE RF
FREE AN TI YR LG
Example: _: ..P LONG 5,7-10.
YR - Publication year:
This field is only populated for 'Literature' documents (PT=L). Use
the PD (Patent publication date) for 'Patent' documents (PT=P).
YEAR - Publication year Limit:
Literature documents added to DWBA since 1994 are not retrievable
using the YEAR ..LIMIT option. To retrieve any documents published
in the period January 1994 to date you MUST use the YEAR= quick
code, e.g.:
1_: BLOOD
2_: YEAR=1994 OR YEAR=1995 OR YEAR=1996 OR YEAR=1997 OR YEAR=1998
3_: 1 AND 2
DE - Descriptors:
Few phrases are hyphenated in DE, so use the ADJ or WITH operators
to link terms.
PA - Patent assignee/corporate affiliation
Depending on the publication type this field contains the patent
assignee (patent type documents - PT=P) or the corporate affiliation
of the author(s) (journal type documents - PT=L).
Search Options:
..SET PLURALS ON and ..SET MEDWORD ON can be selected in DWBA.
Guides:
The guide to DWBA is published in the Biomedical Manual, Berne and
online in the BASE database - search BASE-DWBA.
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